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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,218)
Mouse Secondary Antibodies (14,540)
Rabbit Secondary Antibodies (6,314)
Donkey Secondary Antibodies (4,319)
Rat Secondary Antibodies (1,238)
Sheep Secondary Antibodies (701)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (258)
Pig Secondary Antibodies (78)
Horse Secondary Antibodies (74)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (57)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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7,456 – 7,470 of 72,086 results
7,456

Invitrogen™ Rabbit anti-Rat IgG (H+L) Secondary Antibody, AP

Rabbit Polyclonal Secondary Antibody

Content And Storage 4°C, do not freeze
Target Species Rat
Host Species Rabbit
Conjugate Alkaline Phosphatase (AP)
Applications Dot Blot,ELISA,Immunohistochemistry,Immunomicroscopy,Western Blot
Form Liquid
Isotype IgG
Concentration 1 mg/mL
Antigen Rat IgG (H+L)
Regulatory Status RUO
Purification Method Antigen affinity chromatography
Product Type Secondary Antibody
Formulation TBS with 1mM MgCl2, 0.1mM ZnCl2, 50% glycerol, 1% BSA and 0.01% sodium azide; pH 8.0
Immunogen Rat IgG whole molecule.
Classification Polyclonal
Primary or Secondary Secondary
7,457

Rabbit anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed), Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Hamster
Host Species Rabbit
Conjugate Unconjugated
Applications Chemiluminescence Immunoassay (CIA),ELISA,Flow Cytometry,Immunoassay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunomicroscopy,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 4.5mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Immunoassay
Classification Polyclonal
Immunogen Purified Hamster IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on hamster IgG and light chains on all hamster immunoglobulins. This antibody has been pre-adsorbed against human serum proteins.
7,458

Rabbit anti-Canine IgM Heavy Chain Secondary Antibody, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species Canine
Host Species Rabbit
Conjugate Unconjugated
Applications ELISA,Immunohistochemistry,Western Blot
Form Purified
Concentration LYOPH
Antigen IgM
Purification Method Ion exchange chromatography
Dilution Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000, Immunohistochemistry-Paraffin
Gene Alias AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH
Gene ID (Entrez) 3507
Formulation 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
Classification Polyclonal
Immunogen Dog IgM u mu chain fragment
Reconstitution Reconstitute with 0.1mL deionized water.
Primary or Secondary Secondary
Test Specificity This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Dog IgM and Dog Serum. No reaction was observed against Dog IgG.
7,459

Rabbit anti-Goat IgG (H+L) Secondary Antibody, DyLight 350, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Goat
Host Species Rabbit
Conjugate DyLight 350
Applications Flow Cytometry,Immunomicroscopy
Form Purified
Isotype IgG
Concentration LYOPH
Antigen IgG (H+L)
Dilution Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000
Formulation 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Classification Polyclonal
Immunogen Purified Goat IgG (H&L)
Reconstitution Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on goat IgG and light chains on all goat immunoglobulins
7,460

Invitrogen™ Rabbit anti-Mouse IgG1 Recombinant Secondary Antibody, Alexa Fluor™ Plus 488

Rabbit Recombinant Monoclonal Secondary Antibody

ENCOMPASS_PREFERRED
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Target Species Mouse
Host Species Rabbit
Conjugate Alexa Fluor Plus 488
Applications Immunohistochemistry (Paraffin),Immunocytochemistry
Form Liquid
Isotype IgG
Concentration 1 mg/mL
Antigen Mouse IgG1
Regulatory Status RUO
Purification Method Affinity chromatography
Product Type Secondary Antibody
Formulation Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.3-7.3
Immunogen Purified Mouse IgG.
Classification Recombinant Monoclonal
Primary or Secondary Secondary
Clone 87H11L26
7,462

Rabbit anti-Chicken IgY (H+L) Secondary Antibody, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Chicken
Host Species Rabbit
Conjugate Unconjugated
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgY
Concentration 1.0 mg/mL
Antigen IgY (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1000-1:10000, ELISA, Immunohistochemistry 1:200- 1:2000, Immunocytochemistry/Immunofluorescence 1:200- 1:2000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Chicken IgG (IgY)-heavy and light chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this reacts specifically with chicken IgG and with light chains common to other chicken immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
7,463

Rabbit anti-Opossum IgG (H+L) Secondary Antibody, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Opossum
Host Species Rabbit
Conjugate Unconjugated
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1000-1:30000, ELISA 1:1000-1:30000 (Primary) 1:100-1:500 (For Coating Plates), Immunohistochemistry 1:200- 1:2000, Immunocytochemistry/Immunofluorescence 1:200- 1:2000, Immunohistochemistry-Paraffin 1:200-1:2000
Classification Polyclonal
Immunogen Opossum IgG-heavy and light chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this reacts specifically with opossum IgG and with light chains common to other oposuum immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
7,464

Rabbit anti-Sheep IgM (H+L) Secondary Antibody, DyLight 680, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Conjugate DyLight 680
Target Species Sheep
Host Species Rabbit
Applications ELISA
Form Purified
Isotype IgG
Antigen IgM (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Ovine IgM
Primary or Secondary Secondary
7,465

Rabbit anti-Goat IgG (H+L) Secondary Antibody [Janelia Fluor 525], Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Goat
Host Species Rabbit
Conjugate Janelia Fluor 525
Applications Western Blot,Flow Cytometry,ELISA,Immunohistochemistry,Immunoassay
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Rabbit anti-Goat IgG (H+L) Secondary Antibody was developed against purified goat IgG (H&L).
Primary or Secondary Secondary
7,466

Rabbit anti-Human IgG (H+L) Secondary Antibody, Texas Red, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species Human
Host Species Rabbit
Conjugate Texas Red
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG
Regulatory Status RUO
Purification Method Multi-step
Dilution Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Gene Alias immunoglobulin heavy constant gamma 1 (G1m marker)
Classification Polyclonal
Immunogen Human IgG whole molecule
Primary or Secondary Secondary
Test Specificity Human IgG (H and L)
7,467

MilliporeSigma™ Rabbit anti-Human IgM, Clone: RM121, Secondary Antibody,

Rabbit Monoclonal Antibody

Antigen Human IgM
Regulatory Status RUO
Purification Method Protein A Purfied
Content And Storage Stable for one year at 2°C to 8°C from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Host Species Rabbit
Applications ELISA
Form Purified
Formulation Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Classification Monoclonal
Immunogen Human IgM
Isotype IgG
Primary or Secondary Secondary
Clone RM121
7,468

Rabbit anti-Mouse IgG (H+L) Secondary Antibody [Janelia Fluor 525], Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Mouse
Host Species Rabbit
Conjugate Janelia Fluor 525
Applications Western Blot,ELISA,Immunohistochemistry,Immunocytochemistry/Immunofluorescence,Fluorophore-Linked Immunosorbent Assay
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Rabbit anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with mouse IgG whole molecule in rabbit
Primary or Secondary Secondary
7,469

Rabbit anti-Bovine IgG F(ab')2 Secondary Antibody, Biotin, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Bovine
Host Species Rabbit
Conjugate Biotin
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Concentration LYOPH
Antigen IgG F(ab')2
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000
Gene Alias immunoglobulin heavy constant gamma 1 (G1m marker)
Classification Polyclonal
Immunogen Bovine IgG F(ab')2 fragment
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity No reaction was observed against Bovine IgG F(c).